Which of the following statements best defines DNA torsion during replication?

DNA torsion during replication refers to the twisting tension that builds up in the DNA molecule as the double helix unwinds to allow the replication process to occur. This torque results from the inherent helical structure of DNA and can impede the movement of the replication fork if not properly managed. As the two strands of DNA are separated, they create a positive supercoiling ahead of the fork, which can hinder the progression of helicases and polymerases necessary for replication.

While it is true that some torsion is necessary for the separation of strands, excessive torsion can lead to problems, making the management of DNA torsion critically important during replication. Additionally, the torsion is not utilized as a method of stabilizing the DNA structure, nor is it a process directly involved in DNA repair. Instead, it is primarily recognized as a challenge that needs to be addressed for successful replication, highlighting why the defining statement focuses on the hindrance caused by this twisting force.